Annexin A1 interaction with a zwitterionic phospholipid monolayer: a fluorescence microscopy study.

نویسندگان

  • J Alfredo Freites
  • Shahla Ali
  • Anja Rosengarth
  • Hartmut Luecke
  • Michael B Dennin
چکیده

We present the results of a fluorescence microscopy study of the interaction of annexin A1 with dipalmitoylphosphatidylcholine (DPPC) monolayers as a function of the lipid monolayer phase and the pH of the aqueous subphase. We show that annexin A1-DPPC interaction depends strongly on the domain structure of the DPPC monolayer and only weakly on the subphase pH. Annexin A1 is found to be line active, with preferential adsorption at phase boundaries. Also, annexin A1 is found to form networks in the presence of a domain structure in the monolayer. Our results point toward an important contribution of the unique N-terminal domain to the organization of the protein at the interface.

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عنوان ژورنال:
  • Langmuir : the ACS journal of surfaces and colloids

دوره 20 26  شماره 

صفحات  -

تاریخ انتشار 2004